Reporter
Part:BBa_K191005
Designed by: Le Thanh Tu NGUYEN Group: iGEM09_EPF-Lausanne (2009-10-11)
TRP promoter - RBS - TetR - Term - TetR promoter - RBS - RFP - Term
Part BBa_K191005 is a double-repressor system: there is a Trp promoter in front of the TetR gene, followed by the Tet promoter in front of the RFP gene. This means that it should react both to tryptophan and tetracycline, an allosteric blocker of TetR (for our experiments we used anhydrous tetracycline, ATC).
Usage and Biology
This part permits characterization of part BBa_K191003 as it contains Trp promoter sequence, to which the fused protein LovTAP can bind. For complete genetic circuit, see here.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 43
Illegal SpeI site found at 51
Illegal AgeI site found at 1576
Illegal AgeI site found at 1688 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//function/reporter/fluorescence
emission | 630nm |
input_s | Trp, LovTAP, ATc |
positive_regulators | |
protein_out | RFP |